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The serial position and interval effect of the human odor odour recognition system was tested by Tempo software. pro-resilin-expressing and control flies were trained to associate their odour with sugar in a T-maze (Fig. 7) and the trained flies were tested with their choice. The choice of the resilin-expressing flies was significantly less than the control flies in the first two trials. The interval between the training and the test was set to 10 min and 20 min for the experiments. In Fig. 7, the 95% confidence interval is shown in a logarithmic scale. The choice of the flies was calculated as the ratio of the number of flies in the left box to the total number of flies and it was adjusted to the expected 50% (random choice).

Genetic correlation between the gene expression and behavior was tested by correlation analysis. Correlation analysis was performed using SPSS (version 23.0, IBM Corp., Armonk, USA). In the expression correlation experiment, we had two different types of control: (1) homozygous flies of the same genotype for resilin gene (genetic background) and (2) heterozygous flies for pro-resilin gene (genetic background). All flies were collected and used for the correlation analysis. In the behavioral experiment, we had two different types of control: (1) homozygous flies of the same genotype for pro-resilin gene (genetic background) and (2) heterozygous flies for pro-resilin gene (genetic background). All flies were collected and used for the correlation analysis. The R package corrplot was used to visualize the correlation for both experiments.

The choice of the flies was calculated as the ratio of the number of flies in the left box to the total number of flies and it was adjusted to the expected 50% (random choice). In Fig. 7, the 95% confidence interval is shown in a logarithmic scale. The choice of the flies was calculated as the ratio of the number of flies in the left box to the total number of flies and it was adjusted to the expected 50% (random choice).

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One of the drawbacks to this approach was that we needed to recompile every time we made even a small change to the code. This slowed everything else we were doing down and because we couldn't always have someone waiting for the application to finish building. 827ec27edc